Calculators
Oligo Analyzer
DNA/RNA oligo Tm, GC%, MW & rev-comp.
Base composition
Reverse complement (5′ → 3′)
This free oligo analyzer computes the key properties of a DNA or RNA oligonucleotide for PCR primer design and lab work: length, GC content, melting temperature (Tm) by the basic Wallace rule and a nearest-neighbor-style salt-adjusted estimate, molecular weight, the µg/OD and nmol/OD extinction coefficient, base composition, and the reverse complement. Paste a sequence 5′→3′ and it recalculates instantly.
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